cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota malang,
Jawa timur
INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 15 Documents
 1   2 
Search results for , issue "Vol. 8 No. 1 (2018)" : 15 Documents clear
Antiviral Effect of Pterocarpus indicus Willd Leaves Extract Against Replication of Dengue Virus (DENV) In Vitro Beti Ernawati Dewi; Marissa Angelina; lia meilawati; Sri Hartati; Indah Dwiatmi Dewijanti; Mei Ria Santi; Hidayati Desti; Mirawati Sudiro
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.10

Abstract

Dengue hemorrhagic fever (DHF) is major public health problem in tropical and subtropical areas of the world with lack of approved vaccines and effective antiviral therapies. With no current treatment for illness attributed to dengue virus (DENV) infection other than supportive care, therapeutic strategies that use natural extract was developed. Indonesia have many plants that potential for antiviral drµgs such as Pterocarpus indicus Willd (P. indicus). The objective of this study was to determine the effect of P. indicus to inhibit DENV replication. We used a well-differentiated hepatocytes-derived cellular carcinoma cell line (Huh-7 it-1 cells) to determine and select antiviral activity. The toxicity effects were determined by MTT assay. Then, the suppression of DENV replication was determined by Focus assay. Dengue infected cells with DMSO were used as control. We found that crude extract (Pi), hexane (Pi.1) and ethyl acetate (Pi.2) extract showed strong inhibition with high selectivity index (SI) of 1,392; 285.36 and 168.56 respectively.  Sub fraction of Pi.1 and Pi.2 still showed strong inhibition with high SI.  Further sub-sub fraction of Pi.2 such as Pi.2.12 and Pi.2.12.1 still showed inhibition of DENV replication but there was reduction of SI value. The mechanism experiment of Pi.2.12, we found that Pi 2.12 more profound to inhibit in the post infection stage that entry or pre-infection. We conclude that the sub-fraction of Pi.2.12 has potential antiviral activity against DV infection in vitro. Further studies are still needed to investigate the pure compound of Pi.2.12 that inhibit and have advantages in the future as alternative for treatment of DENV infection.
The Potential of Indigenous Bacteria for Removing Cadmium from Industrial Wastewater in Lawang, East Java Agung Pambudiono; Endang Suarsini; Mohamad Amin
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.11

Abstract

Heavy metals have been used in various areas around the world especially in the industrial sector. Heavy metals contamination is very dangerous for ecosystem because of its toxicity for some organisms. Cadmium (Cd) is a dangerous metal pollutant that can cause remarkable diverse of toxic effects, in particular for humans and animals. The use of bacteria as bioremediation agents has been widely studied because more efficient, less cost, and environmentally friendly strategy. This present study aimed to isolate and identify Cd-resistant bacteria from the industrial disposal site. Wastewater samples were collected from disposal site of agar flour industry in Lawang Malang, East Java. The collected wastewater effluent was analyzed for physicochemical properties. Isolation of Cd-resistant bacteria was carried out using serial dilution. Bacterial isolates were observed and tested for their effects on the content of Cd. The content of Cd was tested daily using Atomic Absorption Spectroscopy (AAS) for seven consecutive days. Data was analyzed using one-way ANOVA (p < 0.05) and Tukey test. Characterization of potential bacterium was performed using bacterial identification kit. Four bacteria isolates have been successfully isolated from the wastewater sample. There was a statistically significant difference between groups as determined by one-way ANOVA (F = 1229.62, p = 0.00). A Tukey post hoc test revealed that all conditions are significantly different from each other. The content of Cd in wastewater sample was statistically significantly lower after taking the A isolate (3.39 mg/L, p = 0.00), B Isolate (1.47 mg/L, p = 0.00), C Isolate (1.15 mg/L, p = 0.00), and D isolate (1.95 mg/L, p = 0.00) compared to the control treatment (5.11 mg/L, p = 0.00). Two of the most potential isolates identified as Pseudomonas flourescens (C isolate) and Enterobacter agglomerans (B isolate).
The Role of Coenzymes on Mercury (Hg2+) Bioremediation by Isolates Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3 Liswara Neneng; Yohanes Edy Gunawan
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.04

Abstract

Mercury pollution is dangerous to health. Previous research was found two potential Gram-negative bacteria for mercury bioremediation, from gold mining in Central Kalimantan, Indonesia.  These isolates were identified as Pseudomonas aeruginosa KHY2 and Klebsiella pneumonia KHY3. Mechanisms of mercury bioremediation had not known yet by these isolates.  This study purposed to test the role of coenzymes on mercury bioremediation by these isolate and to determine the coenzymes best level of mercury bioremediation. Experimental design was Completely Randomized Design in a laboratory.  Treatment factors were coenzymes obtained from vitamins B1, B6, B12, with 6 levels of treatments, included 1 control.  All treatments were done in Luria Broth media that contain 12 ppm of mercury. Mercury was measured by AAS Shimadzu AA-6200. The results showed that coenzymes effect was very significant to improve mercury bioremediation by P. aeruginosa KHY2 and K. pneumonia KHY3.  Supplementation of vitamin B12 in culture media, more enhance of mercury bioremediation compared with vitamin B1 and B6. These result above, indicated the mechanism of mercury bioremediation in both isolates, were the enzymatic process.
Glycerol Utilization as Substrate for Ethanol Production in Escherichia coli Recombinant under an Aerobic Condition Wahyu Suradi Pranata; Prayoga Suryadarma; Djumali Mangunwidjaja
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.14

Abstract

The utilization of glycerol as a substrate for ethanol production in E. coli recombinant harboring ethanologenic gens (PDC and ADHB) under aerobic conditions was investigated. This research was conducted by using E. coli BW25113 which compared the growth characteristics on glycerol and glucose as carbon source. E. coli can grow well on both substrates and it consumed glycerol faster than glucose. On glucose, E. coli occurred overflow metabolism that indicated by high acetate accumulation. Meanwhile, on substrate of glycerol the acetate accumulation could be reduced. Using glycerol substrate on E. coli increased piruvate accumulation, comparing with glucose. Therefore, the growth characteristic of glycerol was more effective. In E. coli ∆pta/pHfdh/pTadhB-pdc utilized glycerol substrate was able to accumulate pyruvate as intermediate metabolite for produce 2.18 gL-1 ethanol.
Tetracera scandens as a Medicinal Plant: Secretory Structures, Histochemistry, and Antibacterial Activity Evi Muliyah; Sulistijorini Sulistijorini; Yohana Cecilia Sulistyaningsih; Mohamad Rafi
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.12

Abstract

Tetrascera scandens, a member of Dilleniaceae, is used for traditional medicine; the stem is utilized by the Anak Dalam tribe of Jambi Province, Sumatera island, Indonesia, to treat diarrhea symptoms. The aims of this study were to identify the secretory structures, histochemical aspects, and the antibacterial potency of T. scandens stem. Histological study of the secretory structures of T. scandens stem was carried out. The species has idioblast cells and trichomes as its secretory structures. Histochemical analysis indicated the substance secreted by T. scandens idioblast cells mainly contains alkaloids, terpenoids, and phenols. Trichomes of T. scandens only contain flavonoids. The antibacterial activity of methanol extracts was tested against Staphyllococcus aureus and Escherichia coli. Different concentration of extracts was tested using the well diffusion method. According to the results, 100 mg/mL T. scandens extract showed the best inhibitory activity with a maximum inhibition zone of 17.7 mm against S. aureus and of 12.5 mm against E. coli. This study provides scientific evidence that the stem of T. scandens has antibacterial activity and justifies its use by the local community.
Fishermen’s Perspective on Herpetofauna: A Case Study from Kuala Tungkal, Tanjung Jabung Barat, Jambi Nia Kurniawan; Anggun Sausan Firdaus; Fitra Arya Dwi Nugraha; Andri Maulidi; Aguung Sih Kurnianto
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.01

Abstract

The population of herpetofauna (Class: Reptilia, Amphibia) is continuously declining throughout the world. Moreover, habitat changes such deforestation drive rapidly to declining process. Human communities play an important role in conserving the herpetofauna by maintaining and improving the harmonious relationship between human and environment. Therefore, these association will strongly support the conservation of herpetofauna and their habitats. This study is aimed to survey the herpetofauna communities, explore the local people perspective on herpetofauna, and the relationship between human and herpetofauna. We found 12 species observed in this survey that included to 3 order of herpetofauna: anura, squamata and testudines. Local people recognized herpetofauna species around them well and do not harm them because of their roles, such as part of nature (39%), bioindicator (33%), myth (17%), and economic value (11%). We also revealed the local knowledge on herpetofauna, as 14 local names and their roles. Despite Kuala Tungkal is a strategic area for the national port development that probably impacts herpetofauna microhabitat, it needs serious attention on herpetofaunal issues.
Toxicity Test on Wedusan (Ageratum spp.) Extract Against Sitophilus spp. (Coleoptera: Curculionidae) Erinus Mosip; Bambang Rahardjo; Ludji Astuti
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tropical condition in Southeast Asia has contributed around 30% of post harvest damage as it provide optimum condition for the growth of Sitophilus spp. pest. Therefore, this study was aimed to investigate the effect of wedusan extract on controlling Sitophilus spp. Growth. Wedusan extract was given in different level of concentrations. The research used the Randomized Complete Design. Wedusan (Ageratum spp.) extract affected the imago mortality of Sitophilus spp. There were two kinds of Wedusan extract used in this study, dried extract (DE) and fresh extract (FE).  Sitophilus imago were put into bottles filled with organic rice and were treated with Wedusan extract. The mortality level was obsered at 24 Hours After Application (HAA), 48 HAA, and 72 HAA. The highest toxicity was found by the treatment of dried extract of wedusan 5 tablets, by LC50 55% on 24 HAA and 82.25% on 72 HAA. Extract of dry matter has higher toxicity effect than fresh extract. The application of wedusan extract is eviromental friendly and specific-range against pest. It is promising for future development as natural product in agriculture area.
The Correlation of Regulatory T (TReg) and Vitamin D3 in Pediatric Nephrotic Syndrome Yunika Nurtyas; Krisni Subandiyah; Loeki Enggar Fitri
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.08

Abstract

Nephrotic syndrome (NS) is an autoimmune disease that correlates to the imbalance of regulatory T cells (TReg). This study was aimed to investigate the effect of vitamin D as adjuvant therapy of TReg population in pediatric nephrotic syndrome. This study was designed randomized clinical trial, double blind, with pre- and post-test control groups involving 15 subjects newly diagnosed with NS. Subjects were divided into 2 groups, namely K1 for group treated with prednisone+vitamin D and K2 group for prednisone treatment only. The population of TReg in peripheral blood mononuclear cells (PBMC) was analyzed using flowcytometry. Vitamin D serum level was measured through ELISA method. Results showed that there was a significant elevation of TReg (independent t-test, p = 0.010) in K1 group, which was higher than in K2 group. The Pearson test in the K1 group showed that vitamin D level was positively correlated with TReg (p = 0.039, r = 0.779).
Levels of 25(OH)D3, IL-2, and C-peptide in Children with Type 1 Diabetes Mellitus (T1DM) Receiving Vitamin D3 Supplementation Tjahyo Suryanto; Harjoedi Adji Tjahjono; Edi Widjajanto
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.06

Abstract

Type 1 Diabetes Mellitus (T1DM) has become a health problem in many countries. T1DM is the consequence of autoimmune destruction process of β cells. There was relationship between vitamin D deficiency with T1DM. The destruction process was caused by an imbalance of pro-inflammatory and anti-inflammatory cytokines. One of the pro-inflammatory cytokines is IL-2. C-peptide examination to see the function of beta cells due to destruction of pancreatic beta cell. Administration of vitamin D3 supplementation still cause controversy and give varying results. This randomized clinical trial was conducted to determine the levels of 25(OH)D3, IL-2, and C-peptide in people with T1DM who received vitamin D3 supplementation. The subjects were 26 children with T1DM, divided into K1 group (received vitamin D3 supplementation) and K2 group (received placebo). The results showed higher levels of 25(OH)D3 in the K1 group and statistically found a significant difference (p = 0.00). Higher levels of IL-2 and lower C-peptide were obtained in the K1 group and no statistically significant differences were found (p = 0.76 and p= 0.26). The insignificant relationship and the negative correlation were found between 25(OH)D3 and IL-2 (p = 0.71; r = - 0.12), 25(OH)D3 and C-peptide (p = 0.59; r = -0.16), also levels of IL-2 and C-peptide (p = 0.13; r = -0.44) in children with type 1 diabetes who received vitamin D3 supplementation. From this study can be concluded that administration vitamin D3 supplementation in patients with T1DM can increase levels 25(OH)D3 significantly. This increase has not significantly lowered levels of IL-2 and increased levels of C-peptide. However, there was an absolute decrease in the rate of slower C-peptide in the supplemented group than in the placebo group.
Ethnobotany of MandailingTribe in Batang Gadis National Park Aswarina Nasution; Tatik Chikmawati; Eko Baroto Walujo; Ervizal Amir Muhammad Zuhud
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.09

Abstract

 Batang Gadis National Park (BGNP) located in Bukit Barisan Mountains, Sumatera Utara. A Mandailing tribe  who lives around the BGNP, has the unique local knowledge, such as processing young stem of rattan (Calamus manan) into pakkat (traditional food) and use rimbang (Solanum torvum) to neutralize toxins. These local knowledge could be lost because it only inherited orally from generation to generation. This study was aimed to reveal ethnobotany knowledge of Mandailing Tribe. The study was conducted in November 2015 in four villages around the BGNP, Sibanggor Jae, Hutabaringin Julu, Pastap Jae, and Botung Villages. Data were collected by interviewing informants in each village as well as the field survey through two approaches, emic and etic. A total of 262 plant species is used by Mandailing Tribe for subsistence and commercial needs. The highest utilization is for food  (106 species), followed by traditional medicines (81 species), firewood (29 species), building materials (35 species), and animal feed (25 species). People also used plant for household appliances, agricultural equipment, art materials, ropes and wrap, and pest control materials. Eme/rice (Oryza sativa) have the highest Index of Cultural Significance (ICS) values. The existence of this species is maintained for its local wisdom. Thus, involvement of  local  community will give great contribution to manage and conserve the BGNP area. 

Page 1 of 2 | Total Record : 15

 1   2 

Filter by Year

2018 2018


Reset
Filter By Issues
All Issue Vol. 13 No. 3 (2023) Vol. 13 No. 2 (2023) Vol. 13 No. 1 (2023) Vol. 12 No. 3 (2022) Vol. 12 No. 2 (2022) Vol. 12 No. 1 (2022) Vol. 11 No. 3 (2021) Vol 11, No 3 (2021) Vol 11, No 2 (2021) Vol. 11 No. 2 (2021) Vol. 11 No. 1 (2021) Vol 11, No 1 (2021) Vol 10, No 3 (2020) Vol. 10 No. 3 (2020) Vol 10, No 2 (2020) Vol. 10 No. 2 (2020) Vol 10, No 1 (2020) Vol. 10 No. 1 (2020) Vol 9, No 3 (2019) Vol. 9 No. 3 (2019) Vol 9, No 2 (2019) Vol. 9 No. 2 (2019) Vol. 9 No. 1 (2019) Vol 9, No 1 (2019) Vol. 8 No. 3 (2018) Vol 8, No 3 (2018) Vol. 8 No. 2 (2018) Vol 8, No 2 (2018) Vol. 8 No. 1 (2018) Vol 8, No 1 (2018) Vol 7, No 3 (2017) Vol. 7 No. 3 (2017) Vol 7, No 2 (2017) Vol. 7 No. 2 (2017) Vol. 7 No. 1 (2017) Vol 7, No 1 (2017) Vol 6, No 3 (2016) Vol. 6 No. 3 (2016) Vol 6, No 2 (2016) Vol. 6 No. 2 (2016) Vol. 6 No. 1 (2016) Vol 6, No 1 (2016) Vol 5, No 3 (2015) Vol. 5 No. 3 (2015) Vol 5, No 2 (2015) Vol. 5 No. 2 (2015) Vol 5, No 1 (2015) Vol. 5 No. 1 (2015) Vol. 4 No. 3 (2014) Vol 4, No 3 (2014) Vol. 4 No. 2 (2014) Vol 4, No 2 (2014) Vol. 4 No. 1 (2014) Vol 4, No 1 (2014) Vol 3, No 3 (2013) Vol. 3 No. 3 (2013) Vol. 3 No. 2 (2013) Vol 3, No 2 (2013) Vol 3, No 1 (2013) Vol. 3 No. 1 (2013) Vol. 2 No. 3 (2012) Vol 2, No 3 (2012) Vol. 2 No. 2 (2012) Vol 2, No 2 (2012) Vol 2, No 1 (2012) Vol. 2 No. 1 (2012) Vol. 1 No. 2 (2011) Vol 1, No 2 (2011) Vol 1, No 1 (2010) Vol. 1 No. 1 (2010) More Issue